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2 CFR 200 does not place a limit on the number of times this may happen. So, barring any applicable Federal statute limitslimitations, or Federal awarding agency discretion, the Federal award may be subawarded as many times as each pass-through entity determines is necessary, as long as each subaward continues to meet the requirements in 200.331. (cf. Uniform Guidance FAQ .331-2 here).
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